Generation of an induced pluripotent stem cell line from an adult male with 45,X/46,XY mosaicism
نویسندگان
چکیده
منابع مشابه
Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells
The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor c...
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Recent studies have demonstrated that embryonic stem cell-like induced pluripotent stem (iPS) cells can be generated by enforced expression of defined transcription factors. The fact that cell fate change is accompanied by changes in epigenetic modifications prompted us to investigate whether chemicals known to modulate epigenetic regulators are capable of enhancing the efficiency of iPS cell g...
متن کاملGeneration of induced pluripotent stem cell lines from adult rat cells.
Supplemental Experimental Procedures Cell culture. Rat bone marrow cells (BMC) were cultured in αDMEM culture medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum. Rat primary ear fibroblasts (PEF) were cultured in DMEM culture medium (Invitrogen) supplemented with 10% fetal bovine serum. Rat iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) in K...
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Peripheral blood mononuclear cells (PBMCs) were collected from 55-year old male patient with a confirmed diagnosis of hemorrhagic Moyamoya disease (MMD). PBMCs were reprogrammed using Sendai virus particles delivering the four Yamanaka factors. A footprint-free hiPSC line was characterized by the expression of pluripotency markers and a normal karyotype. These cells were able to give rise to Em...
متن کاملGeneration of male germ cells from induced pluripotent stem cells (iPS cells): an in vitro and in vivo study.
Recent studies have reported that induced pluripotent stem (iPS) cells from mice and humans can differentiate into primordial germ cells. However, whether iPS cells are capable of producing male germ cells is not known. The objective of this study was to investigate the differentiation potential of mouse iPS cells into spermatogonial stem cells and late-stage male germ cells. We used an approac...
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2018
ISSN: 1873-5061
DOI: 10.1016/j.scr.2018.01.003